Numerical simulations of diamond microfluidic device for the biomolecules electrophoretic separations

Jan M. Lysko¹, Dariusz Witkowski², Damian Obidowski², Anna Karczemska²

¹The Institute of Electron Technology, al. Lotnikow 32/46, 02-668 Warsaw, Poland.
²Technical University of Lodz, Institute of Turbomachinery, ul. Wolczanska 219/223, 90-924 Lodz, Poland.

DOI:

https://doi.org/10.7494/cmms.2009.1.0213

Abstract:

Microfluidic devices known also as labs-on-chip, capillary electrophoresis microchips or micro total analysis systems (µ-TAS) were introduced in 1990’s for the first time. These devices have attracted much attention over the last years. They transfer tiny quantities of samples and reagents, through a system of microchannels and microchambers manufactured on the surface of a small plate. They are made up of different materials, most often of polymers (PDMS, PMMA), glass or silicon. Microfluidic devices are applied in many areas including separations of biomolecules (DNA or proteins), DNA amplification and sequencing, chemical synthesis, single cell analysis, environmental monitoring etc. They often integrate many steps of analysis such as sample preparation, separation and detection on a small, single chip. In this paper we study electrophoresis microchips. Joule heating and its effects, i.e., the temperature growth leading to temperature gradients in microfluidic devices, lead to many problems during chip electrophoresis. Sample band dispersion (low column separation efficiency), reduction of analysis resolution, destruction of thermally labile biomolecules or formation of vapor bubbles are the negative effects of Joule heating. In our research we compare diamond, glass and PDMS microfluidic devices. Microchips of different geometries and of different materials have been analyzed by the Conventor™ software. Diamond reveals exceptionally good electro-thermo-opto-chemical parameters, is very useful in the range of biomolecular separations (such as electrophoresis) and for optical detection methods. Among them, the most important are: the highest-ever thermal conductivity coefficient, good optical transparency, very high electrical breakdown voltage, good chemical resistance and mechanical durability. Diamond microfluidic devices are very advantageous over glass or polymer microfluidic devices commonly used. They dissipate Joule heat much more efficiently because of the highest-ever thermal conductivity coefficient of diamond.

Cite as:

Lysko, J., Witkowski, D., Obidowski, D., & Karczemska, A. (2009). Numerical simulations of diamond microfluidic device for the biomolecules electrophoretic separations. Computer Methods in Materials Science, 9(1), 97 – 103. https://doi.org/10.7494/cmms.2009.1.0213

Article (PDF):

Keywords:

Diamond microfluidic device, Electrophoresis, Numerical simulations, Joule heat

References:

Erickson, D., Towards numerical prototyping of labs-on-chip: modeling for integrated microfluidic devices, Microfluid Nanofluid, 1, 2005, 301-318.

Manz A., Graber N., Widmer H.M., Miniaturized Total Chemical Analysis Systems: a Novel Concept for Chemical Sensing, Sensors and Actuators B: Chem. 1, 1990, 244-248.

Manz, A., Harrison, D., Verpoorte, E., Fettinger, J., Paulus, A., Ludi, H., Widmer, H., Planar chips technology for miniaturization and integration of separation techniques into monitoring systems: capillary electrophoresis on a chip, Chromatogr J., A 593, 1992, 253-258.

Björkman, H., Ericson, C., Hjertén, S., Hjort, K., Diamond microchips for fast chromatography of proteins, Sensors and Actuators, B79, 2001, 71-77.

Björkman, H., Rangsten, P., Hollman, P., Hjort, K., Diamond replicas from microstructured silicon masters, Sensors and Actuators, 73, 1999, 24-29.

Adamschik, M., Hinz, M., Maier, C., Schmid, P., Seliger, H., Hofer, E.P., Kohn, E., Diamond micro system for biochemistry, Diamond and Related Materials, 10, 2001, 722-730.

Khanna, P., Villagra, A., Kim, S., Seto, E., Jaroszeski, M., Kumar, A., Bhansali, S., Use of nanocrystalline diamond for microfluidic lab-on-a-chip, Diamond and Related Materials 15, 2006, 2073-2077.

Müller, R., Schmid, P., Munding, A., Gronmaier, R., Kohn, E., Elements for surface microfluidics in diamond, Diamond and Related Materials, 13, 2004, 780-784.

Hassard, J., US Patent 6752914 – Capillary electrophoresis device, 2004.

Karczemska, A., Sokołowska, A., Materials for DNA sequencing chip, Journal of Wide Bandgap Materials, 9, 2002, 243-259.

Karczemska, A., Ralchenko, V., Lysko, J., Louda, P., Kaczorowski, W., Hassard, J., Poly-diamond device for microfluidic application, Abstract book of the Vacuum and Plasma Surface Engineering – International Conference, Liberec – Hejnice, 2007, 33.

Karczemska A., Diamond Microfluidic devices, Abstract book of the Vacuum and Plasma Surface Engineering, 26th-27th October 2006, Liberec-Hejnice, Czech Republic.

ERA-NET MNT/98/2006, Diamond Microfluidic Devices for Genomics and Proteomics, EU project, 2007-2010.

Wu D., Qin J., Lin B., Electrophoretic Separations on Microfluidic Chips, Journal of Chromatography A, 1184, 2008, 542-559.

Erickson, D., Sinton, D., Li, D., Joule heating and heat transfer in poly (dimethylsiloxane) microfluidic systems, Lab Chip, 3, 2003, 141-149.

Tang, G., Yan, D., Yang, Ch., Gong, H., Chai, Ch., Lam, Y., Joule heating and its effects on electrokinetic transport of solutes in rectangular microchannels, Sensors and Actuators A 139, 2007, 221-232.

Krishnamoorthy, S., Bedekar, A.S., Feng, J.J., Sundaram, S., Need for Simulation-Based Design Analysis and Optimization, JALA, June 2006, 118-127, doi:10.1016/j.jala.2006.02.007.

Cao, J., Hong, F.J., Cheng, P., Numerical study of radial temperature gradient effect on separation efficiency in capillary electrophoresis, International Communications in Heat and Mass Transfer, 34, 2007, 1048-1055.

Karczemska, A., Ralchenko, V., Lysko, J.M., Bolshakov, A., Sovyk, D., Louda, P., Fijałkowski, M., Hassard, J., Diamond Microfluidic Devices, submitted to Diamond and Related Materials, Special Edition of Diamond Conference'2008, Abstract DIAM2008_446.

Ralchenko, V., Nano-and microcrystalline CVD diamond films on surfaces with intricate shape, Nanostructured Thin Films and Nanodispersion Strengthened Coatings, eds, Voevodin, A.A. et al., Kluver, 2004, 209-218.

Coventor inc. Microfluidics Reference, 2008.

Witkowski, D., Obidowski, D., Łysko, J., Karczemska, A., 3D Simulations of Microfluidic Devices, Cieplne Maszyny Przepływowe, 133, 2008, 359-368.